Transformation Of E. Coli With Green Fluorescent Protein

Transformed cells take up a plasmid containing the GFP gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to long wave UV light. For 10 Lab Groups. Kit includes: instructions, BactoBeads™, E. coli GFP Host, supercoiled pFluoroGreen™, ampicillin, IPTG, CaCl2, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petriplates (large), plastic microtipped transfer pipettes, wrapped 10ml pipette (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes. You need: automatic micropipette (5-50μl) and tips, two water baths (37ºC and 42ºC), thermometer, incubation oven (37ºC), pipette pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, long wave UV light