Edvotek A-Maizing Editing: Using Crispr To Improve Crops

Explore cutting-edge biotechnology with this hands-on CRISPR-Cas9 simulation. Students will assume the role of plant geneticists working to develop corn crops capable of surviving and thriving, in a changing environment. This experiment will allow students to develop an understanding of CRISPR-Cas9 applications in the laboratory, cleave DNA, and examine their results after gel electrophoresis. Expt. 210 Hands-On Crispr Cas 9 Simulation Explore this cutting edge technology with minimal equipment and class time requirements Create four guide RNAs (gRNAs), then use BLAST to verify and evaluate their specificity Test all four gRNAs plus control and analyse results using gel electrophoresis